http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2312425-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dea97595058f316a875c28a64efb90da |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
| filingDate | 1997-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_582eae8286a71c1bcddaa0aaf1fd57ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94eaa85c4bda4587fe4630c06269e344 |
| publicationDate | 1997-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-2312425-A |
| titleOfInvention | Transformation of Burkholderia spp. with plasmid by electroporation after culture in the presence of an antibiotic |
| abstract | A process for the transformation of Burkholderia spp . (especially B.pseudomallei ) comprises: (i) culture of the bacterial cells in the presence of an antibiotic at a level at which said cells remain viable; (ii) subjecting said cultured cells to electroporation in the presence of a transforming plasmid. The antibiotic may be kanamycin, preferably in a concentration of 10 to 150 micrograms per ml. of culture medium. Electroporation may be effected using an applied voltage of from 1.4 to 1.8 kV (field strength of from 7 to 9 kV per cm.). The process may be used either to effect transposon mutagenesis or to inactivate genes by insertional mutation. The transformed bacterium may be incorporated into a vaccine, preferably in admixture with a pharmaceutically-active carrier. |
| priorityDate | 1996-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.