http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2299085-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_634715a790f7ef0163a61e9f364bebcd |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 |
filingDate | 1996-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0ff98188cd1685fa22de39eebed41c2 |
publicationDate | 1996-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | GB-2299085-A |
titleOfInvention | Expression of a lipase in Pichia species |
abstract | A DNA molecule comprises: (i) a region encoding a polypeptide which is BSSL, bile salt-stimulated lipase, or a functional variant thereof; (ii) attached to the 5'-end of said coding region, a region encoding a signal peptide capable of directing secretion of said polypeptide from P.pastoris cells transformed with said DNA molecule; (iii) operably-linked to said coding regions of (i) and (ii), the methanol oxidase promoter of P.pastoris , or one which is functionally equivalent. The signal peptide may comprise a Saccharomyces cerevisiae invertase signal peptide. Functional variants include deletion of at least one repeat unit of the undecapeptide of sequence GAPPVPPTGDS. The DNA molecule may be inserted into a replication vector, which is used to transform cells of Pichia species, especially P.pastoris , for the expression of BSSL. |
priorityDate | 1995-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 558.