Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a58aab8ab8ea1b2493e25c88e5bd7338 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b0e3f7195a4b77d4a4a26640e6a4c32c |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1228 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate |
1994-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cbd6c38c6ef613250315f39b904ebf0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee77fe876daa8b43d753e941ff1bf906 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f230a5c38c2d87b9e0dd78e0c083eca3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4862cd54add4afda0256692ffba8e127 |
publicationDate |
1995-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
GB-2285863-A |
titleOfInvention |
Immunoassay for cellular organisms |
abstract |
There is disclosed a method for detecting or quantifying organisms such as Erwinia carotovora atroseptica subspecies (Eca). Wells of a microtitre plate are coated with antibodies which bind Eca antigens. Samples of vegetable material are added to the well along with a a medium e.g. D-PEM (double polypectate) which selectively amplifies the bacterial cells in situ. Following a suitable period for cultivation, a labelled antibody is added to the wells. By parallel testing wells which have been enriched or non-enriched with D-PEM, the presence or quantity of live Eca cells capable of causing potato rot and 20 similar diseases can be established. The multiplication step increases the sensitivity of the test such that 100 CFU/ml can be detected. Also disclosed is the monoclonal antibody 4G4 which is Eca-specific. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2995953-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10527611-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11719692-B2 |
priorityDate |
1993-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |