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filingDate 1993-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0890efb6572ddf3b5da4dc47b1bc9de2
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publicationDate 1995-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber GB-2285445-A
titleOfInvention Protecting nucleic acids and methods of analysis
abstract A selected region of a nucleic acid is protected from attack by a reagent e.g. a nuclease by complexing to the nucleic acid a nucleic acid analogue which hybridises thereto in a sequence selective manner. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilised and its product assayed by protecting a characterising region of the product by hybridisation as above followed by nuclease degradation of unprotected nucleic acid. The nucleic acid analogue is most preferably a polymer e.g. a polyamide, polythioamide, polysulphinamide or polysulphonamide to the backbone of which is a bound a sequence of ligands.
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