| abstract |
A cell based transactivation assay, useful in detecting peroxisome proliferator activity of chemical entities, comprises co-transfecting a host cell with a full-length peroxisome proliferator activated receptor cDNA sequence and a combination of a peroxisome proliferator-responsive element, such as that of the rat acyl-CoA oxidase gene promotor, upstream from a promotor linked to a reporter gene, such as chloramphenicol acetyl transferase, CAT. This assay allows detection of peroxisome proliferator activity while employing a minimal quantity of test compound. Cells comprising such constraints are also claimed. |