| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69ece09bafee7e0c49d1f7951a3faa2c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-44 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6459
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-755
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21069 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-755
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate |
1987-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29f0090e9aec4bb23819f16c39d43ea6 |
| publicationDate |
1988-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
GB-2197321-A |
| titleOfInvention |
Expression vector comprising introns and transformed cells |
| abstract |
Production of a desired heterologous protein comprises constructing an expression vector having a stabilizing sequence downstream of a promoter and upstream of the DNA encoding the desired heterologous protein, transfecting and choosing a particular eukaryotic host cell for said production and culturing the transformed eukaryotic host cell under favorable conditions. The stabilising sequence is an intron of a donor-acceptor-splice sequence, preferably from the immediate early gene of human cytomegalovirus or an immuloglobulin variable region. Vectors can be used for continuous production of factor VIII, t.PA and prorelaxin. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6642028-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2251622-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2251622-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9929848-A1 |
| priorityDate |
1986-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |