abstract |
A process for cultivating anaerobic bacterium, characterized by placing in a gas impermeable packaging bag or an air tight container (i) a culture medium inoculated with said anaerobic bacterium and (ii) a material having oxygen-absorbing property and carbon dioxide- generating property packed in a gas permeable hermetically sealing the gas impermeable bag or the air tight container, absorbing oxygen in the gas impermeable bag or the air tight container to less than 0.1% by volume within 10 hours and generating carbon dioxide in the gas impermeable bag or the air tight container, and maintaining the bacterium in a carbon dioxide concentration of 5-15% by volume and an oxygen concentration of less than 0.1% by volume. |