| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ac40ffdd0f9b7563bab9d5dccaa8a6b3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5375
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-536
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-743 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-537
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-536
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 |
| filingDate |
1981-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
1982-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
GB-2078370-A |
| titleOfInvention |
Binding assays |
| abstract |
A method of carrying out a binding assay (e.g. immunoassay) comprises treating the aqueous reaction mixture, subsequent to the binding interaction, with a reactant (e.g. an enzyme) which interacts preferentially with a labelled version of one or other of the binding reagents when said reagent is either bound in a complex or more usually in the free state, to give rise to a product having solubility properties which are substantially different from those of the original binding reagent or complex, thereafter partitioning hydrophobic substances from the treated reaction mixture into a hydrophobic solvent phase and detecting or determining labelled material present in the hydrophobic phase. The liquid-liquid separation step used replaces the solid phase separation step of previous assays. Also the liquid-liquid separation advantageously separates desired labelled material from luminescently or fluorescently-active contaminants contained in the aqueous sample. There is also described the preparation of LCG(5-H<3>)-tryptophan and RCG-(2,3-H<3>)-propionate. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4629689-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8377686-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4876187-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0840895-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0840895-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8903043-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8701810-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9305395-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8632966-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9097672-B2 |
| priorityDate |
1980-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |