| abstract |
Labile coenzymes such as nicotinamide adenine dinucleotide (NAD) and/or enzymes are stabilised by treatment with an organic solvent in aqueous medium while the pH is adjusted within a range of 6.0 to 8.5. An azide may be added to the solution, not only as bacteriostatic agent, but also as stabilising agent. A second coenzyme, such as adenosine triphosphate, may also be stabilised in the same solution. One or more enzymes, such as hexokinase or glucose-6-phosphate dehydrogenase, may also be stabilised against denaturation in the same solution. The process yields a very stable composition which can be used in assays for biological diagnosis. |