http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1521243-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56ee5285e8e8edccb061046ff20da7a6 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-812 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-096 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 |
| filingDate | 1977-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1978-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1521243-A |
| titleOfInvention | Process for fixing proteins on to carriers |
| abstract | 1521243 Immobilizing proteins BOEHRINGER MANNHEIM GmbH 26 Jan 1977 [29 Jan 1976] 3147/77 Heading C3H Biologically active proteins are immobilized by reacting a compound containing at least two secondary amino groups with a chlorination agent and reacting the product obtained with (a) a protein, or (b) a bifunctional compound containing at least one primary amino group and subsequently with a protein, or at least one bi- or polyfunctional protein reagent followed by reaction with a protein, or a protein/ protein reagent reaction product. Examples are given in which glucose oxidase, glucose dehydrogenase or glycerokinase are immobilized by reaction with various chlorinated polyamide carriers, in the form of granules, powder, the inner surface of a tube, the coated inner surface of a rubber tube, or impregnated clay particles, directly or after reaction of the said substrates with (i) ethylenediamine, or oxalic acid dihydrazide, followed by ethyleneglycol bispropionic acid bis-hydroxy-succinimide ester; (ii) hexamethylenediamine or succinic acid hydrazide, followed by glutardialdehyde; (iii) hexamethylene diamine followed, optionally, by a copolymer of acrylic acid hydroxy succinimide ester and acrylamide or of methacrylic acid hydroxy succinimide ester and methacrylamide. In one embodiment, glucose oxidase is reacted with ethyleneglycol bis-propionic acid, bis-hydroxysuccinimide and then with the chlorinated inner surface of a nylon tube, said chlorinated surface having been reacted with hexamethylene diamine and glutardialdehyde. |
| priorityDate | 1976-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 460.