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filingDate 1977-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1978-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber GB-1516820-A
titleOfInvention Process and reagent for determining total and bound cholesterol
abstract 1516820 Determination of cholesterol BOEHRINGER MANNHEIM GmbH 2 Aug 1977 [29 Oct 1976] 32372/77 Addition to 1429526 Heading G1B [Also in Division C3] A process for the determination of total or bound cholesterol comprises liberating bound cholesterol with a cholesterol esterase and simultaneously or subsequently determining the cholesterol with an NAD- or NADP-dependent cholesterol dehydrogenase obtained from an anaerobic micro-organism or from mammalian liver, for example from Eubacterium sp. ATCC 21408. A reagent for carrying out the process comprises cholesterol esterase, the dehydrogenase, if the determination is not to be made by measurement of NADH or NADPH, means for example 2,4-dinitrophenylhydrazine for the determination of cholesterone, a buffer for providing pH 4À5 to 8À5 and optionally a stabilizing agent and/or a surface active agent.
priorityDate 1976-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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