http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1492899-A

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filingDate 1975-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1977-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber GB-1492899-A
titleOfInvention Method for electroquantitative determination of proteins
abstract 1492899 Electrolytic protein determination ICL SCIENTIFIC 24 June 1975 [23 July 1974] 26681/75 Heading C7B A protein having relatively low anodic electromobility is linked via a bifunctional reagent to a protein of relatively high anodic electro-mobility and the reaction product, being non-gelled and having substantially the mobility of the more mobile protein, is electroquantitatively separated on an electroconductive matrix through which a current is passed. The invention is applied to immunoglobulin determination in blood serum using albumin as the mobile protein and glutaraldehyde as reagent. Other reagents specified are p, p<SP>1</SP>-difluoro-m, m<SP>1</SP>-dinitrodiphenyl sulphone, diethylmalonimidate dihydrochloride and dimethyl adipimidate dialdehyde. The matrix may be agarose and both it and the protein product may be buffered conventionally between pH 7À5 and 8À6.
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