http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1488469-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56ee5285e8e8edccb061046ff20da7a6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-32 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-52 |
| filingDate | 1976-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1977-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1488469-A |
| titleOfInvention | Process and reagent for the quantitative determination of lactate |
| abstract | 1488469 Lactate determination BOEHRINGER MANNHEIM GmbH 2 July 1976 27658/76 Heading G1B Process for the quantitative determination of L-lactate in a sample by reaction with nicotinamideadenine-dinucleotide (NAD) in the presence of lactate dehydrogenase (LDH) with the formation of reduced nicotinamide-adenine-dinucleotide (NADH) and pyruvate, reaction of the latter with glutamate in the presence of glutamate-pyruvatetransaminose (GPT) with the formation of alanine and an α-ketoglutorate in alkaline buffer solution and measurement of the NADH formed to indicate the amount of L-lactate in the sample, wherein the reaction with the glutamate is carried out in the presence of at least 0.4 mol/litre of carbonate or bicarbonate. A suitable reagent for the above process comprises LDH, GPT, NAD and glutamate and is characterized by a content of 0À40-1À2 mol/litre of a carbonate buffer of pH 9À5-10À5. The reagent may also contain sodium azide. The process may also be used to determine the L-alamine content in a sample. |
| priorityDate | 1976-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.