http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1479994-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_84d9adaa7e7ce01ce8020f5c721aba10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-60 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-92 |
| filingDate | 1975-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1977-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1479994-A |
| titleOfInvention | Single reagent for the enzymatic determination of cholesterol and method therefor |
| abstract | 1479994 Determination of total cholesterol ABBOTT LABORATORIES 25 Feb 1975 [4 March 1974] 7904/75 Heading G1B Total cholesterol is enzymatically determined in a serum sample using a single reagent comprising a substantially anhydrous mixture of cholesterol esterase, cholesterol oxidase, horse-radish peroxidase (HRP), a hydrogen donor oxidisable by hyrdogen peroxide to produce a colour, and cholic acid, a conjugate thereof or a salt of either, the cholesterol esters in the serum sample being hydrolyzed to free cholesterol which is then oxidized to # 4- cholestenone and hydrogen peroxide, the hydrogen peroxide in the presence of HRP oxidizing the hydrogen donor which may be o-diamisidine or 4-aminoantipyrine + phenol to form a colour which is measured. The assay of cholesterol oxidase by conversion of cholesterol to cholest-4-en-3-one and measurement of the latter at 240 nm is disclosed. Hydrogen donors other than those mentioned above are 4', 4', 4" methylidynetris (N,N-dimethylaniline), 3-methyl-2-benzo-thiazolinone hydrazone/o-toluidine, 3-methyl-2-benzothiazolinone hydrazone/ N,N-dimethyl aniline, 2, 2' -azino-di-(3-ethylbenzothiazolin sulfonate-6) diammonium salt. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0053692-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0044432-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0091026-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0091026-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0024578-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0044432-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5047327-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4275151-A |
| priorityDate | 1974-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.