| abstract |
1381306 Separating bacterial cells by flocculation IMPERIAL CHEMICAL INDUSTRIES Ltd 20 Feb 1973 [3 March 1972] 9981/72 Heading C6F An aqueous fermentation medium containing bacterial cells is Subjected to either or both of the following treatments viz. raising the pH to 8-11 by alkali e.g. aqueous ammonia, and heating to 50-200C for a short time e.g. 0À5-5 minutes, then the cool medium, at a temperature of 20-30C, is treated with acid to lower the pH to 2-5 and the flocculated cells are separated from the medium. In a preferred procedure, a mixture of sulphuric and phosphoric acids is used and the treated medium, after separation of the flocculated cells, is recycled to the fermentation. Examples are directed to the separation of specified strains and species of bacteria. |