http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1359403-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56ee5285e8e8edccb061046ff20da7a6 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-829 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-824 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-986 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9123 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-978 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-933 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 |
| filingDate | 1972-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1974-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1359403-A |
| titleOfInvention | Creatinine amidinohydrolase and creatine amidinohydrolase |
| abstract | 1359403 Creatinine amidohydrolase and creatine amidinohydrolase. BOEHRINGER MANNHEIM GmbH 4 May 1972 [5 May 1971 (2)] 20767/72 Heading C3H Creatinine amidohydrolase (and creatine amidinohydrolase) may be obtained from micro-organisms, such as the designated strains Alcaligenes sp. WS 51400 and Penicillium WS 90001, which have been cultured in a creatininecontaining medium, by digesting them using high pressure, ultrasonics or a disintegration mill, and recovering creatinine amidohydrolase in purified form from the water-soluble digestion fraction by known methods carried out at a pH above 7À0. The water-soluble digest fraction can be purified by fractionation with polyethyleneimine at a buffer concentration of about 0À1 M and /or by precipitation with 90% isopropanol. Separation of the two enzymes is achieved by adsorption on a weakly basic ion exchanger (specified) at an ion concentration below 0À1 M in a column or batchwise. The exchanger is then washed with 0À1 M buffer to remove non-active proteins, and creatinine amidohydrolase and creatine amidinohydrolase are eluted with an ion concentration of 0À2 M and 0À5 M, respectively. Alternatively, the enzymes may be isolated from solution by salt precipitation of fractionation, e.g. with the use of ammonium sulphate, at a concentration of 2À2 M for creatinine amidohydrolase and 2À7 M for creatine amidohydrolase or the mixture of the two enzymes. |
| priorityDate | 1971-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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