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filingDate 1970-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1973-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber GB-1317271-A
titleOfInvention Method of producing l-glutamic acid by fermentation
abstract 1317271 L-Glutamic acid by fermentation AJINOMOTO CO Inc and SANRAKU OCEAN CO Ltd 27 May 1970 [27 May 1969] 25369/70 Heading C2C L-Glutamic acid is prepared by culturing an artifically induced mutant strain of a parent strain of the genera Brevibacterium, Corynebacterium or Arthrobacter under aerobic conditions on a fermentation medium containing, as a source of assimilable carbon, an alcohol, a hydrocarbon or a carboxylic acid derivative thereof, a source of assimilable nitrogen, minor growth-promoting nutrients and from 50 to 500 penicillin units per ml. of said medium of a substance having penicillin activity sufficient to inhibit but not prevent the growth of said mutant strain until a substantial amount of L- glutamic acid is accumulated in said medium and recovering said glutamic acid, the parent strain being capable of assimilating said carbon source and producing glutamic acid in the absence of said substance, and the mutant being capable of growth on an agar nutrient medium containing said substance in an amount corresponding to more than 5 penicillin units per milliliter. The mutant strain is preferably Brevibacterium ketoglutamicum (ATCC 21533), Corynebacterium hydrocarboclastus (ATCC 21534), or Arthrobacter paraffinens (ATCC 21535). The substance having penicillin activity is suitably benzylpenicillin, α-phenoxyethylpenicillin, phenoxymethylpenicillin, α-phenoxypropylpenicillin, 5 - methyl - 3 - phenyl - 4 - isoxazolylpenicillin or α-aminobenzylpenicillin, or a sodium, potassium or calcium salt thereof, or a mixture of said penicillin derivatives and salts thereof. The carbon source is suitably a (C 6 - C 20 ) alkane or a (C 5 -C 20 ) mono- or dicarboxylic acid.
priorityDate 1969-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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