http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1272025-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e50ade4f744023a537dd76a78af742f1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 |
| filingDate | 1970-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b73f55a8e2b71bdf76678e6be7173176 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acd293cc6b6ebb7739478533c2462aad |
| publicationDate | 1972-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1272025-A |
| titleOfInvention | Method for determining cellular ribonucleic acid distribution |
| abstract | 1,272,025. Preparing microscope samples. INSTITUTUAL PENTRU CONTROLUL DE STAT AL MEDICAMENTULUI SI CERCETARI FARMACEUTICE. April 22, 1970, No.19224/70. Heading G2J. A method of determining RNA distribution in vegetable or animal tissues, particularly during cell mitosis, comprises treating such tissue with methanol and HCL then with uranyl ions, and then with a basic dyestuff of the thionine group. In an example, after fixation, sections of tissue 3-5Á thick are split into two groups, the first of which is immersed in 0À1N HCL at 60 degrees for 20 minutes, washed, then dehydrated and combined with the first group. The sections are then passed through three baths of absolute methanol and immersed in absolute methanol containing 0À8 ml % of conc. HCl for 1 hour at 37‹C. This treatment blocks -CO 2 H and -OSO 3 Me groups present in the acid polysaccharides in the tissues. The sections are washed at least ten times in cool (5-8‹C.) water, immersed in a 0À5-1% uranyl acetate in 30% methanol solution for 1 hr. at room temperature, washed again at least ten times with cool water and maintained for 10 minutes at room temperature in a buffered methanolic solution. The sections are then passed into a freshly prepared solution of 5-10mg % toluidine blue in the buffer and left for 45-48 hours. They are afterwards washed, dehydrated and treated with Canada balsam or a synthetic resin and observed under a microscope. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8301779-A1 |
| priorityDate | 1970-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.