http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1268394-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_10572c2437c733277c1908e4b41b2a32 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-849 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-82 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-82 |
| filingDate | 1969-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b77192d3780a5b1846f2e8b6a30499e |
| publicationDate | 1972-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1268394-A |
| titleOfInvention | Method of purifying l-asparaginase |
| abstract | 1,268,394. Asparaginase. J.K. & SUSIE L. WADLEY RESEARCH INSTITUTE & BLOOD BANK. 6 June, 1969 [7 June, 1968], No. 28710/69. Heading C3H. A method of purifying L-Asparaginase from a mixture containing a ruptured microbial organism which yields L-Asparaginase, comprises the steps of: adding to the mixture containing the ruptured microbial organism a sufficient quantity of a water-miscible organic solvent to precipitate the cell walls and other undesirable constituents of the organism present in the mixture without precipitating the LAsparaginase in the solution; separating the precipitated cell walls and other undesirable constituents from the mixture containing the L-Asparaginase; adding to the supernatant containing the L-Asparaginase a sufficient quantity of a water-miscible organic solvent to precipitate the L-Asparaginase; separating the precipitated L-Asparaginase from the solution; -suspending the precipitate in a buffer solution; and chromatographically separating the LAsparaginase from other constituents which are precipitated with the L-Asparaginase. The solvent may be ethanol, methanol, propanol, isopropanol, dioxane, tetrahydrofurane, or acetone or mixtures thereof with each other and/or with water. The chromatographic separation may be in two stages using an anionexchange and a cation-exchange resin. |
| priorityDate | 1968-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.