http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1258063-A
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-814 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-849 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-847 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-881 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-82 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-82 |
filingDate | 1968-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1971-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | GB-1258063-A |
abstract | 1,258,063. L-asparaginase. SOCIAL SERVICES, SECRETARY OF STATE FOR. 24 Nov., 1969 [23 Aug., 1968], No. 40344/68. Heading C3H. A process for the separation of L-asparaginase from a bacterial culture containing L-asparaginase comprises subjecting the bacterial culture or the bacterial cells derived therefrom to the action of alkali to give a bacterial environment having a pH of above 9À0 whereby a portion of the bacterial cell constituents including L- asparaginase is released in soluble form to give an alkaline solution of L-asparaginase. The culture may be of Erwinia or Serratia spp. and may be treated with KOH to pH 9 to 12À5 to lyse the cells then acidified to pH 3-5 with acetic acid to precipitate inactive protein, the asparaginase being finally isolated by adding ammonium sulphate or an alcohol, or by adsorption on carboxymethylcellulose. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0122080-B1 |
priorityDate | 1968-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.