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filingDate 1967-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5f492484f5e446137656ffe38c483c1
publicationDate 1969-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber GB-1173996-A
titleOfInvention Automatic Liquid Chromatography and Chromatograph
abstract 1,173,996. Chromatography; ion exchange. HITACHI Ltd. 10 May, 1967, No. 21803/67. Headings B1J and B1X. Components of organic mixtures, especially amino acid mixtures, are separated chromatographically by means of a cation exchange resin which has been saturated with a weakly basic metal ion which readily complexes with the components of the mixture, the separated components being detected as the complexes so formed, suitably by means of a colour reaction. Column 1 comprises resin saturated with weakly basic metal ions such as any of Ni##, Cu##, Co##, Cd##, Hg##, Zn##, La### or Y###; the amino acid mixture is introduced through cap 18. Eluent from tank 3, suitably a buffered solution which contains the same weakly basic metal ion as that with which the column is saturated, is pumped by pump 2 into the column. The effluent is continuously mixed with a colour reagent from tank 9 in coil 10 in bath 11 (which may be heated), and the developed colour continuously read by photometer 6 and recorded on recorder 7. Suitable ion exchange resins are those containing sulphonic, carboxylic or iminodiacetic acid groups. In the separation of amino acids, suitable colour-developing reagents are 2-carboxy-2-hydroxy-5'-formazyl benzene, xylenol orange, pyrocatechol violet and ninhydrin.
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