http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1139123-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7ad58d3c0b071069a8ee0cb8c8ba2e8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-087 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-8117 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 |
| filingDate | 1967-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53111505e5560db14d7155952a5e8302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4373d8a1ffcddac93b40df9786f99713 |
| publicationDate | 1969-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1139123-A |
| titleOfInvention | Purification of enzyme inhibitors |
| abstract | 1,139,123. Enzyme Inhibitors. FARBENFABRIKEN BAYER A.G. 22 May, 1967 [26 May, 1966], No. 23654/67. Heading A5B. Proteolytic enzyme inhibitors are purified (concentrated) by contacting an impure aqueous solution of the inhibitor with a waterinsoluble polymer-enzyme combination material whereby at least some of the inhibitor is sorbed by the combination material, separating the combination material from the unsorbed aqueous phase, and desorbing a purified aqueous solution of the inactvator from the combination material, the latter having been formed by contact of a proteolytic enzyme, inhibitable by the inhibitor, with a polymeric material cross-linkable by this proteolytic enzyme. The inhibitor may be trypsin, kunitz or kallikrein inhibitors; the enzyme may be trypsin, chymotrypsin or kall-krein and examples of suitable polymeric material are ethylene-maleic anhydride copolymer, maleic anhydride-vinylpyrrolidone copolymer and methacrylic acid-methacrylic acid-3-fluoroanilide copolymer. In a modification of the process, the combination material is water-soluble and, as the impure aqueous solution, serum containing the inactivator is used. The inhibitor is subsequently desorbed by shifting the pH and/ or modifying the ion concentration and/or displacement by means of competitive inhibitors or enzymes e.g. tryptamine, n-butyl-amine or epsilon-amino caproic acid for trypsin, and/ or by the addition of substances, such as urea, which are capable of loosening or dissolving molecular interactions, possibly by a reversible denaturation of the proteins concerned. |
| priorityDate | 1966-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.