http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1083633-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_25f5e60cca882cd756ee1cd36c3fef16 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-818 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-76 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-76 |
| filingDate | 1964-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1967-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1083633-A |
| titleOfInvention | Improved reagent and preparation thereof |
| abstract | A reagent comprising altered human chorionic gonadotropin (HCG) adsorbed on spherical particles of synthetic resin in suspension having a particle size of 0.07 - 0.90m may be used to determine HCG in urine and hence pregnancy by means of an agglutination test using an HCG antiserum, the adsorbed HCG competing with that in the test sample. The HCG is altered by aging or by heating or by applying digestive enzymatic action and heat to a solution of HCG before adsorption on the particles, or to the reagent. Specified resins are polystyrene and polymethacrylic resins. Borate buffers may be used (in an example calcium chloride is present therein also) and sucrose may be added to the final composition. Details are given of the concentrations and proportions of the resin and HCG employed. As described the HCG is altered by (1) mixing with the resin, heating to 96 DEG C, cooling to 50 DEG C and maintaining thereat for 2 - 3 months (2) mixing with the resin, adding a trypsin solution, heating slowly to 50 DEG C and maintaining thereat for 2-4 weeks, (3) adding trypsin thereto, heating to 37 DEG C and maintaining the temperature for an hour, increasing the pH and adding the resin suspension, heating to 50 DEG C and maintaining thereat for 2 - 4 weeks or (4) mixing with the resin and maintaining at 5 DEG C for 6 months. The specification further describes an agglutination test for determining the suitability of the reagent, using an antiserum of known potency in an aqueous diluent containing imidazoline, HCl, NaCl, sodium salicylate, bovine albumin and ethylmercurithiosalicylate or containing imidazoline, HCl and glycine. An antiserum preparation obtained from rabbits injected with HCG may be similarly tested. |
| priorityDate | 1963-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.