http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1076776-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72a377e9f3de5c48201dff688bb57528 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-816 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6448 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6435 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-68 |
filingDate | 1963-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe12ed56fe2af23811819b06cbcfdd4b |
publicationDate | 1967-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | GB-1076776-A |
titleOfInvention | Process for the extraction of elastase |
abstract | Elastase is obtained from defatted pancreas by extraction with sodium acetate-acetic acid buffer at pH 4.8 to 5 followed by precipitation with (NH4)2SO4 at 48 to 55% saturation. The precipitate is resuspended in sodium acetate-acetic acid buffer and, after demineralization, e.g. by dialysis, it is fractionally precipitated from a water-miscible alcohol/water mixture. The active precipitate is recovered, cold-centrifuged, redissolved in sodium acetate-acetic acid buffer at pH 4.7 to 5 and the solution lyophilized. All operations are carried out at a temperature below 5 DEG C.ALSO:Elastase is obtained from defatted pancreas by extraction with sodium acetate-acetic acid buffer at pH 4.8 to 5 followed by precipitation with (NH4)2SO4 at 48 to 55% saturation. The precipitate is resuspended in sodium acetateacetic acid buffer and after demineralization, e.g. by dialysis, it is fractionally precipitated from a water-miscible alchol/water mixture. The active precipitate is recovered, cold-centrifuged, redissolved in sodium acetate-acetic acid buffer at pH 4.7 to 5 and the solution lyophilized. All operations are carried out at a temperature below 5 DEG C. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0057462-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0057462-A3 |
priorityDate | 1963-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.