http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1015262-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ac40ffdd0f9b7563bab9d5dccaa8a6b3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32064 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32151 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-08 |
| filingDate | 1962-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f98ea39c9b741cb993176454243685a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_150e62b0c8b514d9f5d187ef35662480 |
| publicationDate | 1965-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1015262-A |
| titleOfInvention | Virus culture |
| abstract | A virus cultivation process compriser maintaining a viable culture of cells derived from the baby golden hamster kidney fibroblast cell line designated BHK 21 in a nutrient culture medium, inoculating the culture with a virus to which the cells are susceptible and cultivating the virus in the culture. Suitable viruses are foot and mouth disease virus, Newcastle disease virus, fowl plague virus, arthropod-borne encephalitis viruses, herpes simplex trachoma and influenza virus. BHK 21 is developed from a known baby hamster kidney culture by trypainising the monolayers of cells before they are completely confluent, subculturing every 4th or 5th day with 1/10 to 1/20 of the cell suspension using a medium consisting of 10% unheated calf serum and 10% autoclaved tryptose phosphate broth in Eagle's basal medium modified to contain twice the normal concentration of amino acids and vitamins. After several transfers (30 days), all cells except cell line BHK 21 ceased to grow. These cells were trypsinised, resuspended in a medium containing 20% calf serum and 5% glycerol and cooled to -70 DEG C. After 6 days, the cells were thawed at 37 DEG C. and cultured for a further 35 days to produce fast-growing, stable cells. For the production of vaccines, the viruses, cultured as above, are processed by methods known per, se. |
| priorityDate | 1962-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.