http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-1009420-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dfd617dc43a4c986d2dd81e4cb39d9dd |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 |
| filingDate | 1961-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1965-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | GB-1009420-A |
| titleOfInvention | Improvements in or relating to the serological diagnosis of tuberculosis |
| abstract | Kaolin particles 4 to 6 microns in size are prepared by heating kaolin of Japan Pharmacopoeia or equivalent standard to between 790 DEG and 810 DEG C. for 2 to 3 hours and thereafter pulverizing the treated kaolin in deionized water.ALSO:A reagent for the serological diagnosis of tuberculosis comprises a methanolic antigen solution (A) comprising egg-yolk lecithin and tubercle phosphatide produced by extracting acetone- or heat-killed tubercle bacilli with cold acetone to remove cold-acetone-soluble fat, extracting the residue with methanol, evaporating the methanolic solution to dryness in vacuo, extracting the resulting crude phosphatide with hot acetone to remove hot-acetone-soluble fat, dissolving the residue in chloroform to remove chloroform-insoluble matter, adding acetone to the chloroform solution to precipitate the phosphatide, dissolving the phosphatide in chloroform and reprecipitating with acetone and repeating this procedure to purify the phosphatide; a buffer solution (B) produced by adding E.D.T.A. at a concentration of 0,01-0,05 mol to an 0,2 mol tris-(hydroxymethyl)-aminomethane maleate buffer solution and adjusting the pH to between 6,6 and 6,8 by the addition of 1 N NaOH solution; and a standard kaolin suspension (C) obtained by suspending kaolin particles of size 4-6m produced by heating kaolin of the Japan Pharmacopeia or equivalent standard in an electric furnace at 790-810 DEG C. for 2-3 hours and pulverizing the treated kaolin in deionized water.ALSO:A reagent for the serological diagnosis of tuberculosis comprises a methanolic antigen solution (A) comprising egg-yolk lecithin and tubercle phosphatide produced by extracting acetone-or heat-killed tubercle bacilli with cold acetone to remove cold-acetone-soluble fat, extracting the residue with methanol, evaporating the methanolic solution to dryness in vacuo, extracting the resulting crude phosphatide with hot acetone to remove hot-acetone-soluble fat, dissolving the residue in chloroform to remove chloroform-insoluble matter, adding acetone to the chloroform solution to precipitate the phosphatide, dissolving the phosphatide in chloroform and reprecipitating with acetone and repeating this procedure to purify the phosphatide; a buffer solution (B) produced by adding EDTA at a concentration of 0.01 to 0.05 mol to an 0.2 mol tris-(hydroxymethyl)-aminomethane maleate buffer solution and adjusting the pH to between 6.6 and 6.8 by the addition of 1 N NaOH solution; and a standard kaolin suspension (C) obtained by suspending kaolin particles of size 4 to 6m produced by heating kaolin of the Japan Pharmacopeia or equivalent standard in an electric furnace at 790-810 DEG C. for 2 to 3 hours and pulverizing the treated kaolin in deionised water. |
| priorityDate | 1960-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.