| abstract |
The present invention relates to a method for increasing the sialylation of an Fc fragment, comprising a step of mutating at least one amino acid selected from amino acids in position 240 to 243, 258 to 267 and 290 to 305 of said fragment. Fc, the numbering being that of the EU index or equivalent in Kabat. The present invention also relates to a method for producing a variant of a parent polypeptide comprising an Fc fragment, said variant having an improved sialylation of said Fc fragment relative to the parent polypeptide, which comprises a step of mutating at least one acid amine selected from amino acids in position 240 to 243, 258 to 267 and 290 to 305 of said Fc fragment, the numbering being that of the EU index or equivalent in Kabat. |