| abstract |
The present invention relates to a method for dynamically determining the structural profile of a fibrin clot, reflecting its stability in a biological patient sample, said method comprising the following steps: a) mixing the biological sample, undiluted, with tissue factor or a mixture of tissue factor and tissue plasminogen activator; b) incubating the mixture obtained in step a), and then adding calcium ions in the mixture obtained, to initiate the formation of a fibrin clot; c) measuring the turbidity or optical density of the clot in formation of step b), at least two wavelengths between 450 nm and 850 nm, and for a period of between 1 and 35 minutes; and d) determining the clot structure profile analyzed, expressed as the number of protofibrils, density and radius in c) by the formula τ.λ5 = A [Fg]. (λ2 - B), where T is the turbidity of the clot. a given wavelength λ, [Fg] is the initial mass concentration of fibrinogen, and A and B are proportional coefficients, respectively, to the density and the radius of the fibers constituting the clot. e) comparison of the profile obtained with a control. |