http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-3021330-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4f6be7b5f316f050e9151b3afd8936f7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3819 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-286 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_120558e95b20b1103119f186b402d136 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_703b478a13e52b318cb8132c07fdedf9 |
| publicationDate | 2015-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | FR-3021330-A1 |
| titleOfInvention | METHOD FOR ASSAYING DNA APTAMERS |
| abstract | The present invention relates to a method for assaying nucleic acids of the DNA type, capable of being salted out in a flow liquid of an affinity chromatography column, containing a support on which said DNA aptamers are immobilized, said nucleic acids DNA type representing the entire DNA aptamers, or a fragment thereof, said method comprising the steps of: a) providing a sample of said flow liquid likely to contain DNA nucleic acids released from the support of the column affinity chromatography; b) performing PCR amplification of the DNA nucleic acids contained in said sample, using a reaction mixture comprising (i) a pair of nucleotide non-degenerate sense and antisense primers specific for said DNA nucleic acids and (ii) ) a fluorescent intercalating agent, to obtain an amplification product in the form of double-stranded DNA nucleic acids complexed with said fluorescent intercalating agent; and c) quantifying the DNA aptamers, or fragments thereof, by measuring the level of a fluorescence signal generated by said amplification product and comparing this level of fluorescence to that obtained by a standard range generated by a known concentration of said aptamer DNA. |
| priorityDate | 2014-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.