| abstract |
The subject of the invention is the use of mutated RNA polymerases whose peptide chain is modified, by substitution, or deletion, or addition of at least one amino acid, this modification having the effect of reducing the sensitivity of said RNA polymerases to the ITS contained in the DNA sequence coding for an RNA of interest, for the implementation of a method for producing RNA of interest, from determined nucleotide sequences comprising a DNA sequence coding for said RNA of interest and the transcription of which is placed under the control of a promoter recognized by the wild-type RNA polymerases and the mutated RNA polymerases mentioned above, said method having a production yield of said RNA greater than the yield obtained in the case of use of wild-type RNA polymerases in the presence of the same non-consensual STI as that contained in the DNA sequence coding for said RNA of interest. |