abstract |
The invention relates to a method for obtaining cell populations derived from muscle tissue and their use in the preparation of cell therapy products. It consists in particular of culturing cells previously removed by biopsy on skeletal muscle tissues, to identify the different cell types present at different stages of the culture, to choose the stage of culture according to the desired cell population and to harvest cells at the selected culture stage for the preparation of a cell therapy product. It also relates to cell populations derived from muscle tissue obtained by carrying out the method of which the dominant cell type is CD34 +, CD15 + or CD56 +, or comprises a doubly negative cell type CD56- / CD15- or may comprise more minority cell types. CD10 +, Stro-1 + and CD117 +. |