| abstract |
The invention relates to a recombinant screening and / or cloning and / or expression vector, characterized in that it replicates in mycobacteria, in that it contains - 1) a functional replicon in mycobacteria; - 2) a selection marker; - 3) a reporter cassette comprising a) a multiple cloning site (polylinker), b) a transcription terminator active in mycobacteria, located upstream of the polylinker, and c) a coding nucleotide sequence derived from a gene encoding a protein export and / or secretion marker, said nucleotide sequence being devoid of its initiation codon and of its regulatory sequences. |