| abstract |
The present invention relates to a method for implanting covalent binding sites, of the carboxyl type or derivatives, by oxidation and on a carbon substrate, characterized in that it consists in: - selecting a substrate consisting of at least one thread of carbon comprising at least one carbon fiber and having a diameter less than or equal to 150 micrometers, preferably 100 mum and, more preferably still, 50 mum, - optionally to desize it using an organic solvent, - and to carry out the oxidation, at least in part by electrochemistry, at a redox potential less than or equal to 3 volts by reference to a saturated calomel electrode (ECS), in an aqueous solution of salt and / or acid, titrating at total 0.1 to 2 M, preferably 0.2 to 1 M and, more preferably still, 0.3 to 0.5 M. This process makes it possible to achieve one of the aims of the invention, namely the miniaturization of enzymatic sensors. Thus, the invention also relates to enzymatic micro-sensors obtained by implementing the aforesaid method. |