| abstract |
The subject of the invention is a process for the production of xylitol from D-xylose by the action of the Candida parapsilosis strain ATCC28474, characterized in that it comprises the steps consisting in: 1. cultivating an inoculum of 2 to 5.10 * * 6 cells / -ml of C. parapsilosis ATCC28474, aerobically, at a temperature of 25 to 35 degree (s) C, during the time necessary for the consumption of sugar, at a pH maintained in the range of 3.8 to 5.4, in a fermenter containing: either a synthetic medium comprising 30 to 100 g / l of D-xylose, 2 to 10 g / l of KH2 PO4, 1 to 5 g / l of (NH4) 2 SO4, and 0 , 1 to 1 g / l of MgSO4, 7H2 0, or a hydrolyzate of vegetable raw materials comprising 50 to 80 g / l of D-xylose, in the presence of 0.5 to 3 g / l of yeast extract, in aeration and agitation conditions ensuring an oxygen supply such that the bioconversion of D-xylose into xylitol is ensured, without allowing the reuse of xylitol by the yeast, and 2. isolating the xylitol from the culture medium. Application to the industrial biosynthesis of xylitol. |