| abstract |
In a first step, human milk is subjected to microfiltration through a membrane having a pore diameter of the order of 0.1 to 0.3 mum, to obtain a permeate ndegre (s) 1, which consists of a fraction containing a predominant proportion of alpha-lactalbumin; lactoferrin; serum albumin; peptides; and a negative retentate (s) 1, which consists of a fraction containing a major proportion of lactoferrin, caseins; and higher molecular weight proteins; and in a second step, the permeate ndegre (s) 1 is subjected to ultrafiltration on a membrane having a cut-off threshold of between approximately 80,000 and 300,000 daltons, to obtain a permeate ndegre (s) 2 which consists of a fraction containing a major proportion of alpha-lactalbumin and a minor fraction of proteins and peptides of lower molecular weight, and an ndegre (s) 2 retentate, which consists of a fraction containing a major proportion of lactoferrin and a minor proportion of serum albumin . |