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filingDate 1979-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1980-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber FR-2432171-A1
titleOfInvention METHOD AND KIT FOR USING SEMI-PERMEABLE MICROCAPSULES FOR THE DETERMINATION OF A BIOLOGICALLY ACTIVE AND FREE SUBSTANCE
abstract Method and necessary to detect and measure an active substance in a liquid sample containing the substance and proteins. The sample is incubated with semi-permeable microcapsules containing antibody specific for the substance to be detected and an analogue of this substance which can distinguish it. The membrane of the capsules allows the free substance to pass (which competes with the analog to bind to the antibody) but retains the natural proteins and the antibody. The antibody and the free substance are then separated. Determination of the amount of analog associated with the antibody or present in the rest of the reaction system indicates (by reference to a control curve) the amount of free substance present in the sample. Application: determination of a hormone (thyroxine, cortisol, testosterone or digoxin).
priorityDate 1978-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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