| abstract |
The freeze-dried virus-stabiliser prod is heated at 50-150 degress C esp. 90-125 degrees C for 5-35 mins, pspec. 100 degrees C for 20, mins. Stabilisers are suitably heat- or acid-degraded gelatine, opt. contg. sorbitol or a compsn contg albumin, sugar phosphate and glutamine and are used at 1 mu g-30mg per haemagglutination unit. Viruses are esp. adeno- or myxoviruses esp. Sendai virus strain M34996, measles virus Edmonton strain, influenza strain WRL 55 or infectious canine hepatitis virus. The process leaves the nanogranular layer of the myxovirus intact and vaccines have better storage stability than those produced by chemical inactivation of the virus. |