| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df67e3cd062c59a425c6e6a1b834dc7e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2730-10122 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-063
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-303
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate |
1986-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
1987-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-8706166-A1 |
| titleOfInvention |
A PROCEDURE TO EXTRACT AND PURIFY A PROTEIN FIXED TO CELLS |
| abstract |
METHOD OF EXTRACTION AND PURIFICATION OF PROTEINS FIXED TO CELLS. IT CONSISTS OF ADJUSTING THE PH OF THE SUPERNADANT OF ARTIFICIALLY OBTAINED YEAST CELLS TO 6G0.1, WHICH HAVE PRODUCED THE ALPHA-1-ANTITRYPSIN PROTEIN OR THE SURFACE ANTIGEN OF HEPATITIS B AND WHICH HAVE NOT BEEN ROTONICATED IN THE PRESENCE OF ITSELF; TREAT WITH POLYETHYLENE GLYCOL UNTIL THE SUPERNADANT IS CLARIFIED AND, THEN, WITH A DIVALENT METALLIC CATION, SUCH AS CALCIUM OR MAGNESIUM, OR WITH AMMONIUM SULPHATE AFTER ULTRAFILTRATION. IT HAS APPLICATIONS FOR THE EXTRACTION AND PURIFICATION OF SURFACE ANTIGEN OF HEPATITIS B VIRUS AND ALPHA-1-ANTITRYPSIN PRODUCED BY RECOMBINANT DNA TECHNIQUES. |
| priorityDate |
1985-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |