| abstract |
PROCEDURE FOR PREPARING AN INACTIVATOR, EVENTUALLY FREE OF HEPATITIS VIRUSES AND EVENTUALLY LYOPHILIZABLE, OF THE CL-ESTERASE OF THE COMPLEMENTARY SYSTEM. IT INCLUDES THE FOLLOWING OPERATIONS: FIRST, IT IS PART OF HUMAN PLASMA CONTAINING AND FREE BLOG PROTHROMBIN; SECOND, THIS PLASMA IS TREATED WITH AN ANION EXCHANGER AND THE RESULTING ELUTE CONTAINED IN THE CL INACTIVATOR IS FRACTIONATED WITH NEUTRAL SALTS; THIRD, THE PROTEINS ACCOMPANYING THE CL INACTIVATOR ARE ABSORBED WITH A HYDROPHOBIC ABSORBENT; FOURTH, THE INACTIVATOR CL IS DISSOLVED IN DISTILLED WATER AND A SUGAR IS ADDED TO IT, FOR EXAMPLE SUCROSE UP TO 60 AND THEN THE DISSOLUTION FORMED UNTIL THE TEMPERATURE OF 60 DEGREES IS REACHED AND IT STAYS AT THIS TEMPERATURE DURING 10 HOURS AND LASTLY, THE SUCROSE OF THE DISSOLUTION IS REMOVED, BY PRECIPITATION WITH AMMONIC SULPHATE. |