| abstract |
PROCEDURE FOR OBTAINING POLYPEPTIDES THROUGH BACTERIAL EXPRESSION OF A STRUCTURAL GENE THAT CODES FOR THESE. IT CONSISTS OF THE FOLLOWING STAGES: 1. HAVE A BACTERIAL INOCULANT TRANSFORMED WITH A REPLICABLE PLASMIDIC EXPRESSION VEHICLE, WHICH HAS A DOUBLE HEBRA DNA SEQUENCE, IN PHASE FROM A FIRST END 5 '' OF THE SECOND END 3 '' OF THE SAME ENCODER, A PROMOTING OPERATING SYSTEM; NUCLEOTIDES, A STRUCTURAL GEN. 2. PLACE TRANSFORMED INOCULANT IN A FERMENTATION CONTAINER AND GROW IT IN NUTRIENT MEDIA CONTAINING ADDITIVE TRYPTOPHANE. 3. DEPRIVE THE ADDITIVE BACTERIA TO DEPRESS THE SYSTEM AND CAUSE THE EXPRESSION OF THE PRODUCT TO CODE THE STRUCTURAL GENE. |