| abstract |
PROCEDURE FOR OBTAINING CYTOTOXIC SUBSTANCES. IT CONSISTS OF COPULATING THE A CHAIN OF RICIN THROUGH A DISULFIDE BRIDGE WITH A PROTEIN STRUCTURE SUCH AS AN ANTIBODY, IMMUNOGLOBULIN, OR IMMUNOGLOBULIN FRAGMENTS. AS A STARTING POINT, THE GROSS EXTRACT OF GRAINS OF <<RICINES COMMUNIS>> IS PURIFIED BY CHROMATOGRAPHY ON A SEPHAROSE 4 B COLUMN. THE PURE SOLUTION OBTAINED IS CHROMATOGRAPHY IN A LOW IONIC STRENGTH BUFFER TO WHICH A REDUCING AGENT SUCH AS AS 2-MERCAPTOETHANOL. FINALLY, A P1-SH COMPOUND IS REACTED WITH ANOTHER P2-S-S-X, SO THAT WHEN P1 IS THE RADICAL OF THE A CHAIN OF RICIN, P2 IS AN IMMUNOGLOBULIN, OR AN IMMUNOGLOBULIN FRAGMENT, AND WHEN P1 IS AN IMMUNOGLOBULIN OR ONE FRAGMENT, P2 IS A RADICAL OF THE A CHAIN OF RICIN, WITH X BEING AN ACTIVATING RADICAL. CANCER TREATMENT. |