| abstract |
Procedure for the preparation of immunostimulants and, more particularly, for the extraction of an immunostimulatory agent, from spleen cells of animal origin, Ehrlich ascite cells or bacteria or Bacillus subtilis and Saccharomyces cerevisiae cells, characterized in that make a suspension of these cells or these bacteria with a mixture of solvents that contains a halogenated hydrocarbon, such as chloroform, and an alcohol, such as methanol, in that insoluble fractions are separated, in particular by centrifugation, by that the liquid fraction is evaporated under reduced pressure and at a reduced temperature and that the solid residue is taken up again in chloroform and the chloroform solution containing the mentioned immunostimulatory agent is collected by filtration. (Machine-translation by Google Translate, not legally binding) |