http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2906221-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2016-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c772a30b6b8bb0bd9e58622dd6606d32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7d0b93b929e89dad8d783b0dfbacc15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3453cc0874b8b9233ca32e74314fac55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7096f482fabf17676313e3aa5c21f613 |
| publicationDate | 2022-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2906221-T3 |
| titleOfInvention | Methods for barcoding nucleic acids for sequencing |
| abstract | A method of labeling a nucleic acid in a sample, comprising: hybridizing a target to a first oligonucleotide comprising a universal adapter sequence and a target-specific sequence; extending said first oligonucleotide to generate a first nucleotide sequence; hybridizing said first nucleotide sequence to a second oligonucleotide; extending said second oligonucleotide to generate a second nucleotide sequence; hybridizing said second nucleotide sequence to a third oligonucleotide comprising a binding site for a first amplification primer and the universal adapter sequence, wherein the third oligonucleotide comprises a stochastic barcode, wherein the stochastic barcode comprises a molecular marker; extending said third oligonucleotide to generate a third nucleotide sequence; and amplifying said third nucleotide sequence using a first amplification primer to generate a plurality of fourth nucleotide sequences, wherein each of the plurality of fourth nucleotide sequences comprises said target and the stochastic barcode, wherein target is an mRNA molecule; and wherein the molecular marker comprises a nucleic acid sequence that provides a counter for the specific occurrence of the target nucleic acid. |
| priorityDate | 2015-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 893.