| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2062173a1ab48561d2106fd4b08f4311 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-41
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-732 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-39591
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-3955
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2863
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-32 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-38 |
| filingDate |
2015-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2022-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_126e7363c46a5a6dfb47e60ec75222b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d56eb893c479d142f4f13e684ea0247
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d334a2b26557c0d4df082eee9ecb7a22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f23af6b7e51ced9d747e9bb3d76594d9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec341b9b023e11ee961d4963f969164d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ecef656c9d2a32b0bf76d0c022c48532
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_060a37f2153f87655db3dee255774a4c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c7925677f2db609138bb2b65ea4f5dd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7796f6b471830271097265a66f41b093
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3cd10f4d00cec99a9c21b32c46998af9 |
| publicationDate |
2022-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2893536-T3 |
| titleOfInvention |
Method for preparing antibody by regulating the sugar content of the antibody |
| abstract |
A method for preparing trastuzumab, the method comprising: culturing animal cells expressing recombinant trastuzumab in a medium comprising glycerol at a concentration of 0.5 to 3% (v/v), manganese at a concentration of 20 to 120 μM, and uridine at a concentration of 3 to 10 mM as additives to regulate the sugar chain content of the antibody; wherein the animal cells are selected from: HD201 cell line expressing trastuzumab deposited under no. accession: KCTC 12164BP and Chinese hamster ovary cell line expressing trastuzumab; wherein the culturing step comprises: (a) culturing the trastuzumab-expressing cells in a medium comprising glycerol at a concentration of 0.5 to 3% (v/v) and manganese at a concentration of 20 to 120 mM; and (b) culturing the cells cultured in step (a) by adding uridine at a concentration of 3 to 10 mM on day 5 of culture; and optionally, wherein the method further comprises (c) culturing the cells cultured in step (b) by adding a feeding medium comprising glycerol and manganese. |
| priorityDate |
2014-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |