http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2893100-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24c0923be36ecb9ea9bb2d1c16436f93 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 |
| filingDate | 2016-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90b17e19f6c680d104e6a83fb14a6f6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3373d7233ef1137e024d12d0171ea916 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10f3b88d49f87645f66f516eb00642d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea9031eaa32997049c41277a01c2edb1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fd178b00f475c41583fac7927794845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a98007cbfa6e352231ee3cc72e360bfa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f43aee76283bbb24eba554e5ceeda602 |
| publicationDate | 2022-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2893100-T3 |
| titleOfInvention | In situ detection of nucleotide variants in samples with a high level of noise, and related compositions and methods |
| abstract | A method of in situ detection of a nucleotide variation of a target nucleic acid in a sample of fixed and permeabilized cells, comprising: (A) contacting the sample with a nucleotide variation (SP) probe and a neighboring probe (NP), wherein the SP comprises a target-anchor (SPAT) segment that can specifically hybridize to a region of the target nucleic acid comprising the nucleotide variation and a preamplifier-anchor (SPAP) segment, and in that the NP comprises a target anchor segment (NPAT) that can hybridize with a region of the target nucleic acid adjacent to the SP binding site and a preamplifier anchor segment (NPAP); (B) contacting the sample with an SP preamplifier (SPM) and an NP preamplifier (NPM), wherein the SPM comprises a segment that can bind to the SP and comprises two or more collaborating anchors to SP ( SPCA), and wherein the NPM comprises a segment that can bind to the NP and comprises two or more NP Collaborating Anchors (NPCAs); (C) contacting the sample with a collaborative amplifier (COM), wherein the COM comprises a first segment complementary to the SPCA, a second segment complementary to the NPCA, and a third segment comprising a plurality of segments of anchor to marker amplifier; (D) contacting the sample with a label probe system (LPS), in which the LPS comprises a plurality of label amplifiers (LM) and a plurality of label probes (LP), in which each LM comprises a segment capable of binding to a marker amplifier anchor segment of the COM and a plurality of marker probe anchor segments, wherein each LP comprises a detectable marker and a segment that hybridizes with the marker probe anchor segment of the LM, wherein the aforementioned hybridizations form a signal generating complex (SGC) comprising a target nucleic acid with the nucleotide variation, an SP, an NP, an SPM, an NPM, a plurality of COMs, a plurality of LM , and a plurality of LPs; and (E) detecting in situ a signal from the SGC in the sample. |
| priorityDate | 2015-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 341.