| abstract |
An isolated nucleic acid molecule encoding a natural killer cell immune function receptor chimeric antigen receptor (NKR-CAR), comprising an extracellular antigen-binding domain that binds mesothelin and comprising the amino acid sequence of SEQ ID NO:234 or SEQ ID NO:240; the transmembrane domain of KIR2DS2, and the cytoplasmic domain of KIR2DS2, in which the mesothelin-binding extracellular antigen-binding domain is connected to the transmembrane domain by a hinge domain, in which the hinge domain is selected from group consisting of a CD8 hinge, a GS hinge, an IgG4 hinge, an IgD hinge, a KIR hinge, an NCR hinge, a SLAMF hinge, an FcR hinge, and a LY49 hinge, in which the transmembrane domain and/or the cytoplasmic domain optionally comprise one or more of a KIR D0 domain, a KIR D1 domain, and/or a KIR D2 domain. |