abstract |
A method of identifying a selected phenotype associated with a synthetic modular polypeptide in a cell ex vivo/in vivo, the method comprising: a) introducing a barcoded library of nucleic acids into host cells, thereby generating a heterogeneous population of cells genetically modified hosts, wherein the barcoded nucleic acid library comprises multiple members, each of the multiple members comprises a nucleotide sequence encoding a different synthetic modular polypeptide, the nucleotide sequence comprising: a coding region comprising two or more coding sequences encoding two or more variable modules, wherein the two or more coding sequences are in frame with each other; and a barcode region comprising two or more unique barcodes, wherein each variable module in the coding region is mapped to a specific unique barcode in the barcode region; b) identify a genetically modified host cell within the heterogeneous population that displays the selected phenotype in response to a stimulus; and c) from the identified host cell of (b), identifying and/or quantifying the barcode region by sequencing, thereby identifying the synthetic modular polypeptide and/or module thereof that produces the selected phenotype. |