| abstract |
A heterodimeric antibody comprising: a) a first monomer comprising: i) an anti-CD3 scFv comprising a first variable heavy domain having SEQ ID NO: 397, a first variable light domain having SEQ ID NO: 398 and an scFv linker, and ii) a first Fc domain; b) a second monomer comprising a VH-CH1-hinge-CH2-CH3, in which VH is a second variable heavy domain; and CH2-CH3 is a second Fc domain; and c) a light chain comprising a VL-CL, wherein the VL is a second variable light domain and CL is a constant light domain, wherein the first Fc domain comprises the amino acid substitutions S364K and E357Q and the second Fc domain comprises the amino acid substitutions L368D and K370S, wherein the CH1-hinge-CH2-CH3 of the second monomer comprises amino acid substitutions N208D, Q295E, N384D, Q418E, and N421D, wherein the first and second Fc domains each comprise amino acid substitutions E233P, L234V, L235A, G236del and S267K, where the scFv linker is (GKPGS)4 where the second variable heavy domain and the second variable light domain form an antigen binding domain, and where the numbering is according with EU numbering. |