http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2875538-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_74b7b50944d8e1960440fb1f5440b7fd |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-183 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-4846 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6432 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
| filingDate | 2017-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a1428381606e441dbfbfeda69c2be6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b712c6d530b221bb8e8290386de0d24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d278648f32c1ce834c6fdb69933b42ff |
| publicationDate | 2021-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2875538-T3 |
| titleOfInvention | Preparation of factor Xa derivatives |
| abstract | A method of preparing an expressed polypeptide product from a polynucleotide construct comprising the nucleic acid sequence of SEQ ID NO: 7 or a nucleic acid sequence encoding an amino acid sequence having at least 90% identity sequence with the amino acid sequence encoded by SEQ ID NO: 7, comprising: adding a detergent to a sample containing a polypeptide product expressed from the polynucleotide construct; loading the sample on an affinity chromatograph based on soybean trypsin inhibitor (STI) and eluting the polypeptide with a first elution buffer to generate a first eluted sample, where the loaded sample does not contain an organic solvent; loading the first eluted sample onto a mixed-mode ion exchange chromatograph and eluting the polypeptide with a second elution buffer comprising at least 1M of an inorganic salt to generate a second eluted sample; and loading the second eluted sample onto a hydrophobic interaction chromatograph and eluting the polypeptide with a third elution buffer comprising at least 2mM sodium chloride, thereby preparing a purified sample comprising the polypeptide product. |
| priorityDate | 2016-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 174.