http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2873097-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5d546aa6cbfb52c137af7cafc36bf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24aca9ded2638ea793d05360dde7a4a0 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3604 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0621 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2013-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_518d5570594c0e3e138a750ebca12b5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_556f28e3cd5efd370b7d3357e1712950 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec4caecf560b7808e9a263b0a0e64934 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70696eb800e6daa17ebe9f701ac1db7f |
| publicationDate | 2021-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2873097-T3 |
| titleOfInvention | Method to produce a ciliary marginal zone type structure |
| abstract | A method of producing a cellular aggregate comprising a ciliary marginal zone type structure, comprising a step of culturing a cellular aggregate comprising a retinal tissue in which Chx10-positive cells are present in a proportion of 20% or more of tissue in serum-free medium containing a substance acting on the Wnt signal pathway that can enhance Wnt-mediated signal transduction only for a period before the appearance of a cell expressing the RPE65 gene, followed by culturing the "cell aggregate in which the cell expressing the RPE65 gene does not appear" thus obtained in a serum-free medium containing a serum substitute or a serum-containing medium, each of which does not contain a substance acting on the Wnt signal pathway, wherein the substance that acts on the Wnt signal pathway that can enhance Wnt-mediated signal transduction is selected from a protein that belongs to the Wnt family, Wnt receptor, Wnt receptor agonist and GSK3β inhibitor; and wherein the cellular aggregate comprising a retinal tissue in which Chx10-positive cells are present in a proportion of 20% or more of the tissue is a cellular aggregate that can be prepared by a method comprising the following steps (1) to (3): (1) a first step to subject the pluripotent stem cells to a suspension culture in a serum-free medium containing a substance that inhibits the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) a second stage to subject the aggregate formed in the first stage to suspension culture in a serum-free medium containing a basement membrane preparation, and (3) a third stage to subject the aggregate grown in the second stage to suspension culture in serum-containing medium. |
| priorityDate | 2012-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 246.